We have tried to cover the most popular FAQs regarding our buccal swabs and related DNA products but if you have any questions that are not answered below, please do not hesitate to contact us.
No, we are a manufacturing based company and do not offer these services.
The stabilization liquid becomes cloudy when it is too cool, warm the tube in your hands for a few minutes until the buffer is clear before using the collection device
For optimal collection of saliva RNA prior to isolation, we recommend either the GFX Saliva DNA/RNA collector for short-term storage, or the RFX Saliva RNA collector for longer term storage.
GFX saliva collectors collect and stabilise both DNA and RNA samples. When samples are extracted using the GSPN DNA Saliva-Prep Kits, a minimal quantity of RNA is co-isolated. for best results with the purest DNA we recommend using our GSPN Saliva DNA isolation kits. The GFX collectors are also compatible with RNA isolation kits to purify RNA from saliva samples.
Our GFXA-01 assisted DNA collectors include foam swabs to aid in collection.
For best results the sample should be on the Fill To line, however the kit will still work if the sample is a few millimetres either side of the line.
When analysed by spectrophotometer, samples typically give an A260:280 of >1.7 & A260:230 of >1.5.
DNA can be used for a wide variety of applications, including SNP genotyping, PCR/qPCR, sequencing (Sanger & NGS), microarray, and others.
The expected yield is 10 to 30µg DNA in Adults
Yes, GeneFix is compatible with most kits on the market, however for best results we recommend using Saliva-Prep2.
Samples can be stored at room temperature for up to Five years. They can also be frozen at -20°C or -80°C for longer term storage.
Our swabs have been specifically designed to collect buccal cells or saliva from human cheek linings, they are also used routinely to sample various tissues from other animal sources. If the swabs are to be used for other human tissue sources, the collector assumes liability.
For the most accurate and reliable methods of quantification of DNA we would suggest a fluorometric assay such as Invitrogen™ Qubit. Alternative methods include agarose gel electrophoresis against known concentrations of human DNA and analysis by spectrophotometry.
For smaller infants and animals we recommend using our range of mini swabs that are smaller than the SK range and intended for this purpose.
DNA collected & isolated using Isohelix products can be used for a wide variety of applications, including SNP genotyping, sequencing (Sanger & NGS), microarray, and others.
The isolated DNA is of high quality and suitable for all types of PCR including multiplex and rtPCR reactions.
The expected yield is 1 to 10µg DNA in Adults.
The Isohelix Buccal DNA Isolation Kits are optimized in terms of yield and handling for use with the Isohelix DNA swabs, and use either spin column or precipitation methodologies. We also supply a quick release DNA kit for straight to PCR applications. The swabs are also suitable for use with other DNA isolation protocols and kits.
When stabilised with BuccalFix preservation solution, swab samples remain stable at ambient temperatures for up to two years. DNA preserved using Dri-Capsules remains stable for up to three years.
It is highly recommended to stabilise samples using either BuccalFix, Dri-Capsules, or RapiDri pouches as soon as possible after collection. Our studies have shown that DNA samples from swabs will degrade rapidly without proper stabilisation, making them useless for downstream applications.
The swabs are designed with a small flat head and strong handle allowing pressure to be applied easily to the inside of the cheek. The surface of the swab has been designed to remove buccal cells from the inside of the cheek without causing discomfort.
