Saliva DNA Isolation FAQs
Do you offer paternity/ancestry testing services?
No, we are a manufacturing based company and do not offer these services.
The liquid in the collection tubes looks cloudy, can I still use it?
The stabilization liquid becomes cloudy when it is too cool, warm the tube in your hands for a few minutes until the buffer is clear before using the collection device
Can I isolate RNA from saliva samples?
For optimal collection of saliva RNA prior to isolation, we recommend either the GFX Saliva DNA/RNA collector for short-term storage, or the RFX Saliva RNA collector for longer term storage.
Do DNA samples collected by GeneFix also contain RNA?
GFX saliva collectors collect and stabilise both DNA and RNA samples. When samples are extracted using the GSPN DNA Saliva-Prep Kits, a minimal quantity of RNA is co-isolated. for best results with the purest DNA we recommend using our GSPN Saliva DNA isolation kits. The GFX collectors are also compatible with RNA isolation kits to purify RNA from saliva samples.
What if the subjects I am looking to collect from have difficulty producing saliva at will?
Our GFXA-01 assisted DNA collectors include foam swabs to aid in collection.
Will the kit still work if a smaller or greater volume of saliva than specified is collected?
For best results the sample should be on the Fill To line, however the kit will still work if the sample is a few millimetres either side of the line.
What purity of DNA will I expect to see when using GSPN to isolate GeneFix saliva samples?
When analysed by spectrophotometer, samples typically give an A260:280 of >1.7 & A260:230 of >1.5.
What downstream applications can DNA from GeneFix saliva collectors be used for?
DNA can be used for a wide variety of applications, including SNP genotyping, PCR/qPCR, sequencing (Sanger & NGS), microarray, and others.
What yield of DNA will I expect to get per 1 ml of saliva?
The expected yield is 10 to 30µg DNA in Adults
Can kits other than Saliva-Prep2 be used to isolate DNA from GeneFix collectors?
Yes, GeneFix is compatible with most kits on the market, however for best results we recommend using Saliva-Prep2.
What temperatures should GeneFix samples be stored at, and for how long?
Samples can be stored at room temperature for up to Five years. They can also be frozen at -20°C or -80°C for longer term storage.
Swab DNA Isolation FAQs
Can buccal swabs be used to collect DNA from other sources too?
Our swabs have been specifically designed to collect buccal cells or saliva from human cheek linings, they are also used routinely to sample various tissues from other animal sources. If the swabs are to be used for other human tissue sources, the collector assumes liability.
How can I measure the amount of DNA isolated?
For the most accurate and reliable methods of quantification of DNA we would suggest a fluorometric assay such as Invitrogen™ Qubit. Alternative methods include agarose gel electrophoresis against known concentrations of human DNA and analysis by spectrophotometry.
Are swabs suitable for use with children or animals?
For smaller infants and animals we recommend using our range of mini swabs that are smaller than the SK range and intended for this purpose.
What other downstream applications can the DNA be used for?
DNA collected & isolated using Isohelix products can be used for a wide variety of applications, including SNP genotyping, sequencing (Sanger & NGS), microarray, and others.
Is the DNA suitable for PCR reactions?
The isolated DNA is of high quality and suitable for all types of PCR including multiplex and rtPCR reactions.
What yield of DNA will I get from a swab?
The expected yield is 1 to 10µg DNA in Adults.
How do you isolate the DNA from the swab?
The Isohelix Buccal DNA Isolation Kits are optimized in terms of yield and handling for use with the Isohelix DNA swabs, and use either spin column or precipitation methodologies. We also supply a quick release DNA kit for straight to PCR applications. The swabs are also suitable for use with other DNA isolation protocols and kits.
How long is DNA stabilized for when preserved with BuccalFix/Dri-Capsules?
Is stabilization necessary for swab samples?
It is highly recommended to stabilise samples using either BuccalFix, Dri-Capsules, or RapiDri pouches as soon as possible after collection. Our studies have shown that DNA samples from swabs will degrade rapidly without proper stabilisation, making them useless for downstream applications.
Are the Isohelix DNA swabs easy to use?
The swabs are designed with a small flat head and strong handle allowing pressure to be applied easily to the inside of the cheek. The surface of the swab has been designed to remove buccal cells from the inside of the cheek without causing discomfort.