We were delighted to see that two recent, high-profile studies looking at the effects of spaceflight on astronauts’ microbiomes chose Isohelix buccal swabs to collect their valuable samples.
In the Nature paper, “Longitudinal multi-omics analysis of host microbiome architecture and immune responses during short-term spaceflight,” published by Tierney et. al., (2024), the team used Isohelix swabs to collect samples from three microbial ecosystems (oral, nasal, and skin), before and after a 3-day mission to space.
Using paired metagenomics and metatranscriptomics alongside single-nuclei immune cell profiling, the team characterized microbiomes before, during and after spaceflight. The study revealed shifts in the microbiome and immune response during short term spaceflight; although most microbiome alterations were transient, longer-term shifts were observed in the astronaut’s oral microbiomes.
Another Nature paper, “Spatial multi-omics of human skin reveals KRAS and inflammatory responses to spaceflight”, Park et al (2024), also looked at the effects of short-duration spaceflight on the skin. The study applied a wide range of -omics methods, and as part of this study, Isohelix swabs were used to collect samples of astronauts’ skin microbiomes. Metagenomics and metatranscriptomics analyses were then performed on the skin swab samples. A key finding was that post-flight samples showed significant up-regulation of genes related to inflammation and KRAS signalling across all skin regions.
The results from these two important studies can help guide spacecraft design and response countermeasures for astronauts on future missions, and we are excited to see Isohelix products being used to expand our knowledge in this area.
Isohelix swabs feature a unique swab matrix and quick-release surface that maximises nucleic acid yields. Swab batches are ethylene oxide treated and routinely tested for human DNA contamination, making them ideal for capturing precious nucleic acid samples.
CLICK HERE TO FIND OUT MORE ABOUT ISOHELIX BUCCAL SWABS FOR NUCLEIC ACID COLLECTION OF ORAL AND SKIN MICROBIOME SAMPLES
Isohelix also offer stabilization reagents for buccal swabs that offer long term sample stability prior to isolation