FAQs

FAQs

We have tried to cover the most popular FAQs regarding our buccal swabs and related DNA products but if you have any questions that are not answered below, please do not hesitate to contact us.

 


Swabs FAQs

We have tried to cover the most popular FAQs regarding our buccal swabs and related DNA products but if you have any questions that are not answered below, please do not hesitate to contact us.

1. Are the Isohelix DNA swabs easy to use?
The swabs are designed with a small flat head and strong handle allowing pressure to be applied easily to the inside of the cheek. The surface of the swab has been designed to remove buccal cells from the inside of the cheek without causing discomfort.

2. Is stabilization necessary for swab samples?
If there is a delay of more than a day or two between collection and processing of samples, there could be significant degradation of DNA. In this case, for best results samples should be stabilized during transit and storage.

3. How long is DNA stabilized for when preserved with BuccalFix/Dri-Capsules?
Samples can be kept stable for up to 2 ½ years at room temperature in BuccalFix buffer, or 3 ½ years using Dri-Capsules

4. How do you isolate the DNA from the swab?
The Isohelix Buccal DNA Isolation Kits are optimized in terms of yield and handling for use with the Isohelix DNA swabs, and use either spin column or precipitation methodologies. We also supply a quick release DNA kit for straight to PCR applications. The swabs are also suitable for use with other DNA isolation protocols and kits.

5. What yield of DNA will I get from a swab?
The expected yield is 1 to 10µg DNA in Adults.

6. Is the DNA suitable for PCR reactions?
The isolated DNA is of high quality and suitable for all types of PCR including multiplex and rtPCR reactions.

7. What other downstream applications can the DNA be used for?
DNA collected & isolated using Isohelix products can be used for a wide variety of applications, including SNP genotyping, sequencing (Sanger & NGS), microarray, and others.

8. Are swabs suitable for use with children or animals?
For smaller infants and animals we recommend using our range of mini swabs that are smaller than the SK range and intended for this purpose.

9. How can I measure the amount of DNA isolated?
For the most accurate and reliable methods of quantification of DNA we would suggest a fluorometric assay such as Invitrogen™ Qubit. Alternative methods include agarose gel electrophoresis against known concentrations of human DNA and analysis by spectrophotometry.

10. Can buccal swabs be used to collect DNA from other sources too?
Our swabs have been specifically designed to collect buccal cells or saliva from human cheek linings, they are also used routinely to sample various tissues from other animal sources. If the swabs are to be used for other human tissue sources, the collector assumes liability.


Saliva FAQs

1. What temperatures should GeneFix samples be stored at, and for how long?
Samples can be stored at room temperature for up to Five years. They can also be frozen at -20°C or -80°C for longer term storage.

2. Can kits other than Saliva-Prep2 be used to isolate DNA from GeneFix collectors?
Yes, GeneFix is compatible with most kits on the market, however for best results we recommend using Saliva-Prep2.

3. What yield of DNA will I expect to get per 1 ml of saliva?
The expected yield is 10 to 30µg DNA in Adults

4. What downstream applications can DNA from GeneFix saliva collectors be used for?
DNA can be used for a wide variety of applications, including SNP genotyping, PCR/qPCR, sequencing (Sanger & NGS), microarray, and others.

5. What purity of DNA will I expect to see when using GSPN to isolate GeneFix saliva samples?
When analysed by spectrophotometer, samples typically give an A260:280 of  >1.7 & A260:230 of  >1.5.

6. Will the kit still work if a smaller or greater volume of saliva than specified is collected?
For best results the sample should be on the Fill To line, however the kit will still work if the sample is a few millimetres either side of the line.

7. What if the subjects I am looking to collect from have difficulty producing saliva at will?
Our GFXA-01 assisted DNA collectors include foam swabs to aid in collection.

8. Do DNA samples collected by GeneFix also contain RNA?
GFX saliva collectors co-isolate a minimal quantity of RNA when used with GSPN Saliva-Prep Kits. When using other isolation methods, degraded RNA may be co-isolated with DNA. Therefore for best results we recommend using our saliva isolation kits.

9. Can I isolate RNA from saliva samples?
For optimal isolation of RNA we recommend our New RFX Saliva RNA collector.

10. The liquid in the collection tubes looks cloudy, can I still use it?
The stabilization liquid becomes cloudy when it is too cool, warm the tube in your hands for a few minutes until the buffer is clear before using the collection device

11. Do you offer paternity/ancestry testing services?
No, we are a manufacturing based company and do not offer these services.

For further information, please view our Product Range listed or email info@isohelix.com.